It is the oldest sekvenirovaniya RNA
The researchers successfully extracted and sequenced RNA of the wolf of the Pleistocene epoch, the remains of which 14 300 years has lain in the permafrost of Siberia, writes the Chronicle.info with reference to Focus.
DNA stores information for thousands of years, while the RNA “working copy” of genes breaks down much faster. “The researchers of ancient DNA have previously been reluctant to undertake for RNA, because it is generally more unstable and prone to enzymatic degradation. But after the success in sequencing of RNA from ancient plant material, we assumed that in a well preserved specimen of the animal, frozen in the permafrost may be enough material for sequencing RNA,” says Oliver Smith from the University of Copenhagen.
The researchers took RNA from liver tissue of animal and restore its structure, comparing with RNA wolves of the 19th and 20th centuries, to find common characteristics. To date, RNA that frozen wolf is the most ancient of all, which was actually sequenced. In second place — RNA of maize 700 years ago. Previously, scientists were able to extract RNA from the frozen remains of a man had lain for 5000 years in the ice of the Tyrolean Alps, however, restore its structure failed.